Category Archives: Apologetics

Spirit

i do not know if i have ‘spirit’
i want spirit
if i did have spirit then my life would be complete
i am open — but also somehow also self-closed to spirit
i try to listen to spirit
i listen to the spirit of El Elyon
the unknowable
the incalculable
the infinite
the eternal
but if i ever understand that spirit
it will no longer have spirit-power
since what i comprehend can never be awesome

Desmosome-mitochondrial tethering: just what I see

I found this particular electron micrograph which has two mitochondria and a desmosome in pretty good orientation to look at any periodicities or patterning in the substructure. It seemed to me that the central line of the desmosome, and the cadherin molecules which create it and the links to the plasmalemma (which have been modeled with molecular models) could fit best as a “sprint” type association.  Those of you who are old enough to remember the plastic hair combs that looked like wishbones arranged in parallel that one could use to sort of tie up a pony tail, will recognize this flexible structure.  I found, or should say “think i found” a similar type of density in the cadherin molecules in the center of this desmosome (diagrammed as that side by side “wish bone” array.  Interestingly, cadherins were mentioned to one group to come in a dimer which would very easily form this springy wish bone structure….  and listed the “half wish bone” as one option as a molecular model of the desmosomal cadherins. One of those articles has a great 3D image which doesn’t look exactly like what i see, but is close found here    – and another is this title Studer, Daniel & M Humbel, Bruno & Chiquet, Matthias. (2008). Electron microscopy of high pressure frozen samples: Bridging the gap between cellular ultrastructure and atomic resolution. Histochemistry and cell biology. 130. 877-89. 10.1007/s00418-008-0500-1.
. I actually don’t like the idea of calling the arrangement “untangling desmosomal junctions with knots” as i think if they were knots….there would be no perfectly wonderful order that can easily be seen with ordinary transmission electron microscopy. It is also very appealing to have flexibility within these junctions as the wishbone (wish bone) alignment would afford…. that would be just really fun. electron microscopy mitochondria desmosome junctionSo here is the same electron micrograph as yesterdays post but the mitochondria are actually fuzzed green in a separate layer.  Red dot=a ribosome, approximately 27 nm in diameter. The absolutely regular arrangement of cadherins is seen in the middle of the desmosome…. no knots, no tangles, no mess….  just regularly spaced. This micrograph is not retouched to emphasize anything…. it is just the way it looks on the negative. The plasmalemma of the two cells is not in exact cross section and so it is fuzzed. The span between densities both on the plasmalemmal sides and the center densities of this desmosome work out to be about 1/13-14nm.  I have not put that together with any models yet and in the image below just sized the molecular structure according to the TEM, not using the actual size (just using the shape).

electron microscopy mitochondria desmosome junction
So in the second figure you can see an area that i enlarged, cut and pasted in photoshop, and enhanced in contrast and colored purple  and ADDED what i think are the lines of the wishbone arrangements of the cadherins.  Densities (increased in contrast here) work out to about 1 for every 13-14nm.

Into two enlargements (one the purple box above and another with the molecular model of a cadherin dimer, copied and mirrorred, fit really nicely into what might be a flexible, stretchy springy type portion of the desmosome.  Just a thought….  ??

electron microscopy mitochondria desmosome junction
6110_5080_mouse_f_liver_36,200x_4x

 

Tiny minds create a tiny god

“There is no avoiding the fact that we live at the mercy of our ideas This is never more true than with our ideas about God.”
This is a quote by Dallas Willard. I think it speaks to why we need an “omnipotent, omniscient, omnipresent “entity” [0-0-0] to be the goal and center inspiration of our lives.
It is pretty clear to me that what we know, we do not worship, and so the mere act of thinking one understands what the 0-0-0 is then we move that entity out of our worship zone into the “trash” of our own understanding and out ideas about that 0-0-0 in reality become limited and tainted by our own ability to think. In this very sense man “creates” a god that becomes fraught with human emotions, and biases, and unconscious prejudices, and fear and judgement.
The ideation of god is critical to how mankind sees himself and others.
When our god(s) are selfish, indulgent, vengeful, we get what we have now on the planet, and it is not pretty. (there are wonderful thinkers too, and for them i am grateful, it allows me to have an 0-0-0 being so far above and beyond that it becomes comforting. at the same time, i run the risk of being too far removed that the 0-0-0 doesn’t function for me.)
I have come to think there is a “boundary of life/self/0-0-0” in all of our minds. I see it in a very commonplace way. Some people think that their sphere of existence only includes their automobile, they keep it clean and polished but through their trash out the window onto the streets.  There are others with a slightly larger sphere of “life/self/0-0-0” which includes their home and family. They keep their house tidy and their families cared for. The real thinkers in my humble opinion, are those who think of “life/self/0-0-0” as being infinite. These are the world changers for the better because they revere (worship is a tainted word) the entirety of existence and are NOT apt to violate or trash anyone, any group, or anything in the cosmos.  Kudos to those who can think beyond the tiny sphere that they occupy, they with their own microbiome.