Perfluorodecyliodide: 267 days post infusion, crystal-type-inclusions

Ultimate order, the cell

NEG – 9713
BLOCK – 3775
SPECIES – mouse
TREATMENT – 10% IPFD (perfluorodecyliodide) 5% F68Two bottom images show a periodicity in the “ends” (i presum that is a “growing end”) of these perfluorodecyliodide inclusion crystal-like inclusions)

If one looks at a nearby ribosome in the middle picture you can judge the approximate width of the rhythmic extensions (so something between 20 and 40nm ???).

Find the list of perfluorocarbon emulsions used in a retrospective study, including IPFD.

DURATION – 267 days
DOSE — 100cc/kg, sac 5/08/1974
TISSUE – liver
CELL – likely hepatocyte or kupfer cell
FIX – Karnovsky’s fix, Os04, Etoh
EMBEDMENT – EPON
STAIN – UrAcetate/PbCitrate
TEM – Zeiss 10
MAG – 25000
KV – 60
ENLARGEMENT – 4x
EDITING: photoshop, 4px gaussian blur, dodge and burn,

Open Access Retrospective: Perfluorooctylbromide, liver, mouse, 4 days after ip infusion

Legacy Perfluorocarbons, PFC diagrams, Ultimate order, the cell, , , , ,

Open Access Retrospective: Perfluorooctylbromide, liver, mouse, 4 days after ip infusion

perfluorooctylbromide, legacy perfluorocarbon, artificial blood, TEM, phagocyteThis cell (a phagocyte) in liver has many electron lucent droplets of PFOB in the phagolysosomes: the dose of PFOB was high, and only 4 days have passed.  Nucleus in upper right mitochondria are present, but the fluorocarbon inclusions are massive.

NEG – 8739
BLOCK – 26701
SPECIES – mouse
TREATMENT – perfluorooctylbromide (20% PFOB / 10%F68)
DURATION – infusion ip 02/17/1984, sac at 4 days
DOSE — 43cc/kg
TISSUE – liver
CELL – kupfer cell or phagocyte
FIX – Chick fix, PO4, OsO4 (prob 1%) ET, PO
EMBEDMENT – likely Spurr
STAIN – UrAcetate/PbCitrate
TEM – Elmiskop 1a/V pole piece
KV – 60
MAG – 10,000
ENLARGEMENT – 4x
EDITING: dodge and burn in photoshop to even contrast

Open Access Retrospective: Perfluorodecyl iodide, liver, mouse, 267 days after infusion

Ultimate order, the cell, , , ,

Summary retrospective and ball and stick structure of perfluorodecyl iodide.

Perfluorodecyl iodide, liver, mouse, 267 days after infusion shows that there are crystaline-like inclusions, perfectly electron lucent like the “footprints” of other perfluorochemicals leave during processing, and there are growing ends and branching, one notable example in this micrograph is the cross section (round) and adjacent small crystalline-like protrusions oriented perpendicularly.

legacy perfluorochemicals and artificial blood, perfluorodecyliodide

NEG – 9700
BLOCK – 3775
SPECIES – mouse
TREATMENT – 10% IPFD (perfluorodecyliodide) 5% F68
DURATION – 267 days
DOSE — 100cc/kg, sac 5/08/1974
TISSUE – liver
CELL – likely hepatocyte
FIX – Karnovsky’s fix, Os04, Etoh
EMBEDMENT – EPON
STAIN – UrAcetate/PbCitrate
TEM – Elmiskop 1A, V
KV – 60
MAG – 17,500
ENLARGEMENT – 4x
EDITING: none

Gemini and Copilot have been so fun to work with, and helpful.

Perfluorodecyliodide crystaline-like structure in phagolysosomes

Electron micrographs of liver, Legacy Perfluorocarbons, Perfluorodecyl iodide, Ultimate order, the cell, , ,

This particular image of perfluorodecyliodide crystaline-like structure in phagolysosomes shows the direction of growth, and in some cases a very tiny periodicity in the “ends” of those structures.

NEG – 9719
BLOCK – 3775
SPECIES – mouse
TREATMENT – 10% IPFD (perfluorodecyliodide) 5% F68
DURATION – 267 days
DOSE — 100cc/kg, sac 5/08/1974
TISSUE – liver
CELL – likely hepatocyte or kupfer cell
FIX – Karnovsky’s fix, Os04, Etoh
EMBEDMENT – EPON
STAIN – UrAcetate/PbCitrate
TEM – Zeiss 10
MAG – 50000
KV – 60
ENLARGEMENT – 4x
EDITING: none

Verge of a Dream:

Prose and Poems,

You sit deeply in the leather chair,
the parchment light, like the
horizons’ moon
you are a hundred miles away
an unmoving eternity
across an unparted sea.
I walk through alleyways
and like the one red
rose that finds its way.
I hope to find you.
These are the corners
and keyholes. The vanishing point, the
sweet spring hills that have
banished winter’s light.

rlb 8/18/2000