Peak 5 is a very consistent low thin peak that follows after the broad peak adjacent to the glycosylation peak. See previous blog entries for position and width of this peak (which I have always (at least 99% of the time) given the color code of bright pink.  First set of numbers calculates parameters WITH the missing values as 0. second (far right) set of parameters is calculated from all plots in this dataset but WITHOUT the missing values as 0, they are eliminated.  Plots below the top image are those two variations (with (top row) and without (bottom row) missing values), for the four individual dodecamers studied so far.  The best data comes from using the N of four, mean peak 5 height from each of the dodecamers. Measurements are grayscale 0-255 on the Y axis.