Number of peaks along hexameic arms of SP-D:  More dodecamers added to this dataset shows that the number of peaks using AFM images published previously by Arroyo et al, continues to hover around the 15 peaks/hexamer mark.  This is surprising in a way since i deliberately chose the first few images as the “best” and most clearly defined images of SP-D and expected the number of peaks detected (by all filters and functions) to decline, but in fact it did not. This mean is a summation of all 24 hexamers from all processing apps and all molecules examined to date. Figure below these data are those for each dodecamer.

This actually is supportive of the fact that there are two very regularly seen very small peaks between the glycosylation peak, is very thin, and does not demonstrate a great difference in height compared to valley.  And also,   the peak just before what could be a group of flopping CRD domains, with a small neck domain peak just before and between those three large dense consistent ‘multipeak’ peaks seen by AFM.  A peak that would likely be the neck domain sometimes occurs in about half of the trimers.  This sometimes “detection”, is also similar to the intermittent visualization of a very tiny peak just between the N term peak and the glycosylation peak.

Ablve is a summar of all the image filters, and all the signal processing functions that have been gathered for the first 12 dodecmers.  Not all filters and f unctions were applied to all images, but the settings on the filters and functions have become a stable set.

Below, each dodecamer (named and color coded) is given a repeat of the all inclusive statistics (image above) but also divided into two groups (my peak counts from the original images) my peak counts from the plots obtained (all the same pixel width for a segmented line through the hexamer arm) and all image filtering and signal processing.