I guess after many hours of looking at SP-D dodecamers i have really come to the conclusion that there are four peaks on the LUT (look up table grayscale plots – using ImageJ2 (FIJI)) on each side of the N termini peak.

The AFM cover images of Arryoy et al are significantly larger than other AFM dodecamers.(likely due to some miscalculation by the cover editor in the overall size of the cover image) The t-value is -20.52378. The p-value is < .00001. The result is significant at p < .05. The shadowed images (Lu et al, and Hartschorn, et al,) are significantly smaller than AFM dodecamers (excluding the cover images of Arroyo et al). The t-value is 3.50439. The p-value is .000279. The result is significant at p < .05. Just with very few shadowed images analyzed, AFM would appear to create larger dimensions. Leaving out the cover images of Arroyo et al, and just using AFM, and giving all authors equal representation, the dodecamer is 140.67 nm +/ 7.66