1 to 12 grayscale values are going to be considered background in this particular image (8.37+4.7, N=24, so the outside limit would be 13 grayscale units – 13/255 (5%). This value will be used in the duplicate plots of a single hexamer of the dodecamer I have labeled 127-Sup fig 4 which was published by Arroyo et al. I do not know if there is another analysis i can apply to these images. peak height, subtracting any peaks that do not exceed the height of mean background peaks, nm width (standardized), and sq nm.
The CRDs in this particular image are about 140 sq nm.