Segmented line traces of AFM multimer arms – does enlargement of the image matter? See image below and check for yourself whether the plots of a routine view size (top two plots are replicate of an image imported into ImageJ and traced along one of the many arms of this protein). Third plot and image are one ctrl+ increase in magnification, and fourth plot and image are two ctrl+ increases in image magnification in ImageJ. The differences in the trace are so small, just as likely due to variations in the mouse or pen excursions as to to be meaningful differences in plotting details of the brightness.

Just by way of identification of the processing (in gwyddion) of this multimer  it is a 300ppi remake of a tif, opened in gwyddion and filtered from 25 – 255 range  – 7.