Image processing, signal processing, citizen scientist counts – all significantly different peak counts for SP-D

Image processing, signal processing, citizen scientist counts – all significantly different peak counts for SP-D.  I am trying to find concensus here… LOL, all three methods produced results in which there is no concensus.

One arm of one molecule of surfactant protein D measured to find the best method for detecting peaks (literally hundreds of times), show that a method needs to be chosen that the researcher finds a best fit for the images.  A really bad take on a truism about how we see ourselves, might go like this “the peaks that will be, are the peaks I see”. Column colors denote the image processing programs used for the analysis. In the center set, the image processing programs are given on the right, and the signal processing programs (so many variations on the settings not listed there) are the green and yellow colors) statistics are in the third column in each set. N= for image, signal and citizen scientist peak counts is the number of “trimers”, and the mean peak count from the N term to the CRD.  The image that shows the trimer arms marked is HERE.