Comparisons between granule and adjacent cytoplasmlooking for hexagonal or bouquet structures in the alveolar type II cell RER profiles that might be a surfactant protein, such as SP-A. Image below has two portions of cytoplasm outlined (same size), one at tangential areas of a cisternal body – aka – surfactant protein granule in the RER of a type II cell, and the other in nearby cytoplasm.  The redish tinge is created with the eraser tool in photoshop, where I added transparency around areas which appeared to be hexagonal structures with a central dot.  The central dot seems to be an important morphological portion of these hexagons.  See the cytoplasmic rectangle (as opposed to the rectangle from within the granule) to compare whether this might be random, artifact, or possible a reflection of the fixation (yes it is artifact, tell me something new) changes in this protein (I am calling surfactant protein A). There is a difference, I have not made an extensive comparison between intra granule, and extra granule cytoplasm and the general occurrence of 20-40 nm hexagonal structures.  Someone could certainly do that. It is a granule from ferret 9855_23349_#17). Judge size by ribosomes (@27nm) see the red outlines of prominent hexagons in the square within the cisternal body (granule) and count them again in the rectangle over cytoplasm (also enhanced with red as mentioned above).