A549 cell in apoptosis: RNAi knockdown of C9orf82 induced apoptosis in A-
549 cells. Huge granular center of the nucleolus with very little differentiation or dense fibrillar area is seen in middle right part of the micrograph (purple) and a part of the nucleolus with tiny light fibrillar centers and only a small amount of the dense fibrillar component. A portion of the nucleolus is off to the left near a nuclear pore. The cytoplasm of this cell has some RER that does have ribosomes, mostly near the nuclear membrane, and only three sort of not to clear mitochondria exist, one of which is apposed to an nuclear pore. There is very little condensed chromatin around the periphery of the nucleus, mostly euchromatin which is very coarse. Some perichronatin granules, and a little bit of interchromatin granule cluster (speckles) looking like a baseball cap above the circular structures of the nucleolus. Within, perhaps is something dense (don’t know whether to call this a paraspeckle or not (the location within the speckle fits this description). Nuclear chromatin and nucleolus purple, nucleus itself (which includes the interchromatin granule area) orange. 18272_78932_a549_4_si67_apoptosis