The offset shadowing of an electron micrograph (or other photograph) sometimes helps to delineate where the main features are in that photo.  Here the original photo is duplicated, inverted, and 1 pixel by 1 pixel, moved vertically (down) to reveal more detail.  The image is perfectly flat upon beginning, since the images are aligned, and inverse of each other, but the shadow become obvious as the image moves.  This banded protein, which i hope is surfactant protein A or at least mostly SP-A has piqued my interested for 30 years, but I havn’t made/had time to study it.  Here is a video that shows the shadows coming and going, and also marks some of the banding seen in this intracisternal protein.