I manually changed the values from 0-255 on each of the pixel determinations that I retrieved from photoshop (previous post) to make these peaks not valleys.

Original AFM image (one of the two double arms of a dodecamer – CRDs on each end N terminus in middle) on top, sliced and diced and straightened and exported image for Photoshop and pixel determination (which determines – more or less_ the width of the various regions of the SP-D arms, and 3D plot then in excel. and plot from ImageJ, and measurements in NM and grayscale (labeled here luminance – which it probably should not be called).