Platinum shadowed images (with type 1 collagen) of SP-D (referenced so many times on this blog). A portion of one image with LUT plots as 4 lines (I also did 2 rectangles and 2 diagonals to see whether there was a directionality to the way the grains fell during shadowing). First little peak measured against the 135nm SP-D dodecamer (quickly judged by a diameter intersecting at least three of the four CRD) in the same micrograph shows the grains to be about 8 nm in width. I will measure each of these peaks and see whether there is too much variation to use such micrographs to determine peaks and valleys in actual dodecamers. Same image top to bottom, but the yellow line indicates where the LUT plots were made, they correspond well to the plots.