Comparison of LUT plots with different image processing programs has morphed out of my desire to determine how many peaks are along the collagen-like domain of SP-D dodecamers.

I have used half a dozen or more processing programs and many many variations on blurs and medians and sharpens and masks and there is great consensus in the shape of the LUT plots so it is pretty easy to suggest that what looks nice, when compared to an original plot is pretty much going to reflect what is actually present in the image.  Case in point in the figure below where an image (screen print from the actual manuscript (oft used Arroyo et al) processed in coreldraw x5 vs photoshop 2021 really show astounding similarity. The differences in the peak heights are a result of HSL and contrast maniuplations but this doesnt change the data. Diameter of the dodecamers is in inches derived from the original images, and must be reasured against the original bar marker dimensions of 20nm given in the original publifcation (0.69 inches relative to the 5.22 here) making the diameter of this particular dodecamer around 150nm.