Bitplanes-images were obtained as a filter in corel photopaint to visualize LUT peaks along the trimeric arms of SP-D (color>transform>bitplanes with slider) (original image by Arroyo et al).  It has become quite clear that there is a lot of image processing that can be done to AFM images, and with an honest approach, very little of it changes what appears in the original image.

This gif animation was made in GIMP using png files (exported from corel photopaint x5 and sized and edited in corel DRAW x5 to add the arrows that point to three distinct peaks along the collagen like domain of one of the SP-D trimers– in this case to the left of the glycosylation  and N termini peaks ).  While it is garish, the data are real. If you look at the LUT plot (made in ImageJ) from the same SP-D dodecamer in the previous post you will see the three peaks, in their typical increasing height (left to right) as those areas marked by arrows in this animation.

The tiniest (also previously undescribed) peaks that I am pretty sure exists can be seen like  “blips” on either side of the central N termini peak (on the more vertical hexamer).