Daily Archives: June 14, 2019

Half an arm of SP-D dodecamer 3D LUT plot

Having fun with this. It does show the width of the AFM micrographs better than taking just a single line or a thin rectangular plot of a straightened SP-D dodecamer arm.  I like the way the obvious thinning of the height measures work over the collagen like domain peaks, as well as the spread of the trimers where the CRD sort of fan out.  The collagen-like-domain (labeled peak I is not only the tallest of those three peaks, but the broadest as well and may include part of the coiled coil neck?  Any comments are welcome. The N terminus peak is on the right hand side, and has a value very close to 100% in this grayscale plot (using photoshop picker tool at 1 nm2 intervals (thousands of them) and excel to plot the image.  It should be reversed (100 – 0) to make the image inverted. This goes further than the blog yesterday which was just really the CRD.