Peak counts of a single surfactant protein D molecule (an AFM image): signal processing programs: Lag Threshold Influence were measures put into a “batchprocess” app by Aaron Miller which allows selection of different parameters for LTI, and processing on many excel files at once.  The parameters of Lag Threshold and Influence were adjusted, and peaks found in the images (same ones used for finding peaks in other signal processing programs, also listed in a previous post).  This program normalizes x and y and esports those excel files to a new folder using the L T I settings chosen. I ran peak finding on many molecules other than this particular one, where there are only a few samples.  Charts below.  L T and I change peaks counted, and like Octave Ipeaks function, one can choose the input statements to create a count you like.  This is not exactly what I wanted to see with signal processing, but it is what I have come to understand, is what i get. It comes back to using common sense with the image, and then using the programs for automation.