Peak counts of a single surfactant protein D molecule (an AFM image): Citizen scientist counts

This quick look at what the perception of peaks might be in unbiased individuals was an interesting side-trip. Two instances of KNOWN bias on the plots accounted for the smallest peak count and the largest peak count. Haha… I did not expect that.  I represent a reasonable biased count.

I have counted peaks on this same image hundreds of times (actually 274 times – one or two times with the mode of signal and image processing (not every single time, but obviously many many times) which means i counted the original images (pixelated and rough, to the most processed, gaussian blur and limitrange, and the counts with some programs which added rediculous numbers of variations (one example would be “roughen-inside” using Inkscape) and others that eliminated some detail. My peak counts from the exact two plots given to the citizen scientists differ considerably from theirs. I counted the gaussian blur 10px and limitrange 100-255 image as separate peak counts before, and at the same time as that the image was being used by another imaging or signal processing program for peak counts. The mean and standard deviation of my counts are variations in my own observations, which over two years have changed.

My variously obtained peak counts: 1) from images only, 2) from counting plots from image and signal processing programs 3) from graphing out the peak widths from plots obtained from processing.