Grayscale values(0-255) the y axis. Glycosylation peak was detected by image and signal processing algorithms 100% of the time, so there is a single value recorded for the valley (immediately lateral to the “tiny” peak – keeping in mind that this molecule has bilateral symmetry beginning in the center at the N termini junction peak. so many images of this molecule have been posted on this blog that they shape of the AFM images is easy to see.