I went back over the AA sequence and remade this audio and added the visual of the SP-D song. It didn’t change significantly, just some very obvious audio indications of where the collagen-like domain is. I find this so fun. You can see the drone of glycines while you listen to the audio in the image below, and it might be also present in the coiled coil of the neck… remember that the signal peptide accounts for the first 20 notes.