Daily Archives: April 23, 2018

Perfluorooctylbromide: in rabbit hepatocyte

Rabbit liver, two hepatocytes revealed in this electron micrograph. Three possible, one definite, inclusion left over from the infusion of perfluorocarbon, from my notes it looks like perfluorooctylbromide (19.4%PFOB 5%F68), sacrifice dates show that it was more than one year affter infusion.  The mitochondria are pseudocolored green, the microbody purplish, the PFOB inclusion complete round (the density of PFOB is greater than water, which I surmise is the reason these structural footprints are completely round and empty) and the  very tidy layer of proteins around the perfluorocarbon droplet and bounding membrane are blue.  A small (less than 100nm) droplet is typical for the remnants of infusion of the more desirable perfluoochemical blood substitutes. This particular droplet does not show a “black cap” of enzymes which often accompany the droplets. Nice little desmosome between these cells and a bile canaliculus barely seen on the right hand middle.  Golgi in the lower cell at bottom with another microbody (peroxisome – which i should have colored as well.  My recollection is that infusion of some perfluorocarbons was accompanited by proliferation of peroxisosomess (Neg 5890_block_4783_28000x.