Daily Archives: May 24, 2019

Little changes in LUT plots with manipulation of original SP-D images

Well, it seems pretty clear that the LUT plots gathered in ImageJ really are not affected much by the manipulation and scrambling around to make them readable in a program that doesn’t have a lot of line drawing flexibility.  I am convinced that the smallest amount of care will produce quite repeatable results in the LUT plots.  These images are photoshopped, cut, centered, rotated, saved and resaved as RGB or BW images, and the plots don’t really get too different, one to the other. This is good news i guess, hopefully it will be useful to someone studying SP-D
ON THIS HALF arm of a multimeric molecule (n=14) it still looks like the N terminus (left and a portion of the N terminus ring in fuzzyballs) will be the tallest peak (whitest – brightest – most luminous – lightest —still looking for that word to describe the digital whiteness) at or around 240 on the scale of 1-256. After that a close by and very consistent tall but not too wide peak, then possibly 2 or 3 small peaks and finally the carbohydrate recognition domain (not as high a peak as found for this domain – (whitest – brightest – most luminous – lightest level for the multi-lobed CRD).  Bottom two images of SP-D are photoshop images and NOT REAL., Modeled however after the top three images are of one arm which came from a single fuzzyball. (fuzzyball 1 arm 1)