Daily Archives: December 10, 2019

SP-D probably close to 150nm diameter in dodecamers and fuzzyballs

The AFM images all taken (including the cover image which had a micron estimate in the cover description — which was probably a little off since it was larger than the images in the actual manuscript by Arroyo et al. so that is disconcerting) still brings all images in this particular paper to a mean of just about 150nm diameter — (as measured by the diameter of a circle intersecting the majority of CRD at the periphery of the molecules (at least 3 in dodecamers and most in a fuzzyball). This is the same measure that was found previously. It seems painful to want these measurements to be precise, but it is necessary, in my opinion, to have a reasonable standard size to measure peak widths and distances along each of the trimeric arms.

 
Cover n=31 Figure 1 n=13 Figure 4 n=8 Figure 6 n=14 (just pH 7.4)
178.7+/9.7nm 140.32+/5.7nm 133.90+/4.34nm 152.56+/8.3nm