Four new little luminance peaks in SP-D: revisited — this is an AFM image from Arroyo et al which has been examined way beyond anything that is important, perhaps. That is countered with that in using this image with as many different programs with their various transform algorithms I have found some convincing evidence that there is a very nice little luminance peaks between the N termini juncture of an SP-D dodecamer and the purported glycosylation site (which is the big peak adjacent to the N termini.

Three arrows below point to three tiny luminance peaks that showed up after taking the image from their figure 4A (supplement to their manuscript) and doing a 2D continuous wavelet transform (Gwyddion) and a Step Line correction (Data process>Data correction>step line correction). Presumably this would be a symmetrically arranged event so there should be four, but chance and circumstance always play a role.

An additional thing that is apparent is that in this particular dodecamer there is a depression in the peak (which in most images (including this one in its unprocessed state) does not have a depression in the middle of the N termini junction.