Apoptosis seems to cause a real reduction in the chromatin exclusion zone of hepatocytes.  The area is very small on either side of the nuclear pores. This would have to be functional but whether it relates to something changing about the nuclear basket portion of the nuclear pore complex, or about the chromatin, or about some other proteins that are part of the exclusion zone is up for debate. These pores are from nuclei which appear to be undergoing apoptosis, showing lots of peripheral chromatin, a little different in texture than regular condensed chromatin and an euchromatin space which is a little denser and more populated with interchromatin granule clusters than a non-apoptotic cell would be. These cells would be from livers of animals either never rescued or removed from NAC. Pore complexes are about 120nm in their widest dimension and there is another (taken from a n of 5 random pore and their exclusion zone images) another 55nm of exclusion on either side. This is in agreement with a larger selection of chromatin exclusion zones measured in both Gclc ko and 14CoS ko mice… previous posts on this site, which was 48nm and 50.9nm.

Similar small chromatin exclusion zones are found in cells in liver in eminent apoptosis in the 14CoS null mice when they are NOT rescued with NTBC.