It seems there are no big break through numbers looking at the SP-D dodecamer LUT plots from different techniques, this one was all about the actual micrographs and a blended transparency built from images of 24 individual (unbiased selection) of imges (24 arms, 12 dodecamers — measured from CRD to CRD on the opposite side). THe gray scale image and the LUT plot from that image is the result of 24 semitransparent images centered according to the N terminus bright peak and the lateral sides determined by the end of the CRD LUT peaks. Nothing was done to change the position of the smaller but clearly evident, peaks between the N and CRD.  The flattened transparencies were plotted using ImageJ2 (FIJI) and the plot is overlying the image and clear peaks and brighter areas correspond. It seems that four peaks (if you count the extended thickening and brightening of the region just before the CRD, which might be the “neck” there are 4 peaks along the length of the collagen like domain and the neck domain. The reasons for these peaks in brightness are not known, except for the taller and brighter peaks both symmetrical and lateral to the N termini peak.

Image=top row below. Plots and measures of the distances between the valleys of the peaks on one trimer section of the transparent overlays is shown on the left.  This set unfortunately does not quite add up to the width of the whole dodecamer (145 nm) determined by curved – lines with nodes, and also with a diameter touching four of the CRD (see previous posts). Something to work on still.