THis peak has the closest to registering two peak(s) within the larger element of a whole CRD peak area.
THis peak width (just under 17 nm is close to what has been seen before.
Number of peaks within the CRD whole peak depends clearly upon the path of the segmented line used to trace that end of the SP-D trimer.
Daily Archives: July 16, 2022
Four SP-D molecules: peak 7 width and subpeaks
This is an often hidden portion of the SP-D trimer, as it can be hidden under the CRD domain which can flop around and lie in different positions during processing. It seems that this peak will be something around 5 nm (top figure) depending upon how the CRD domains have obscured it. It might not have any subpeaks (the mode for this peak is 1 and it seems reasonable for this relatively tightly wound helical neck portion of the trimers (bottom figure).
It will be interesting what adding four more measurements to this dataset will confirm or refute. It is nice that the small N of four (composed of 50 to 100 separate grayscale plots of trimers (from a tracing of the hexamer) after image and signal processing, show some consensus. 