Measurements of areas surrounding the nuclear pore: hepatocyte


Some measurements (see previous post for comparison data), might be useful in a relative sense to others looking at nucleus, nuclear architecture, and nucleolar architecture.

This particular image is unretouched tangential section of an hepatocyte nucleus (17529neg73229block, anm203,wcii -Gclc 28d).  There are numerous nuclear pores sectioned tangentially in this image, about 35 of which are there 15 or so in sufficient detail to demonstrate a density within the center of the nuclear pore demarcating the site that pre-mRNA and larger particles are ushered through (both ways) (about 44%). Compare this micrograph with a previous post of a similar tangential section for parallel measurement.  They are not identical, but close. Curley red lines demarcate polysomes.

In this micrograph the pore to pore distance is n=34 x=482+177nm, the distance from the outer part of the nuclear pore to adjacent chromatin is n=97, 62.7nm +/-32nm, and the distance between the 19-20 nm beads on a string in the chromatin next to the chromatin exclusion area is n=39, 46nm +/- 13nm. The actual measurements are shown at right (green=pore center to pore center), grey=chromatin exclusion areas, that is the distance between the edge of the nuclear pore and the adjacent chromatin, grey, and purple, the distance between the 20nm beaded-periodicity found in the pore-adjacent chromatin.

electron micrograph hepatocyte measurements of nuclear pore and exclusion area and chromatinelectron micrograph hepatocyte measurements of nuclear pore and exclusion area and chromatin