Stub tail monkey hepatocyte desomosomal-mitochondrial tether: more

There are several nice things about this micrograph of a desmosomal mitochondrial tether (i.e. tethered via the intermediate filaments of the desmosome). One nice thing is the very rigid, and parallel membrane-leaflet quality of the plasmalemma where there is the desmosomal protein complex, and it also exhibits a very even and well defined separation of the outer and inner leafelets of the plasmalemma (at least this is what i think is shown in the micrograph below).  I have marked the two leaflets on the cell membranes on the adjacent cells with black lines. There are obvious periodicities within the inner and outer membrane leaflets too… not withstanding the density of the lead citrate and uranyl acetate stains and the quality of fixation) which seems to present overall patterning in the entire micrograph. I have marked the extracellular lines (which are not likely to be random as they appear, but more likely to be organized attached wishbone shapes (mirrored vertically and copied horizontally) shown in an earlier post but the lines here have been drown over actual densities in the extracellular (intercellular) space of the desmosome. Green blobs mark periodicities within the two leaflets of the plasmalemma, and the pink blobs mark the densities which are extracellular but likely adjacent, maybe even part of (like the cadherins in desmosomes) those proteins. (green blobs are INTERcellular, the pink blobs are within the plasmalemma (transmembrane). Electron micrograph on left is unretouched, on right, contrast and brightness enhanced and vector overlays show densities which can be matched to image on the left. This stub-tail monkey hepatocyte came from an animal that received two test doses of perfluorodecalin (a very small amount) a year prior, which in my experience has nothing to do with these tethers. Shown immediately below the outer leaflet of the plasmalemma is what appears to be a regular compartmentalization (shown as rectangles) likely representing a highly structure arrangement of plakophilin and plakglobin and maybe some desmoplakin and a bit of vimentin.  The number of densities (green or pink, and likely connected parts of the cadherin dimers, turn out in this micrograph to be about 9 nm…  a little close than found in mouse micrographs so far.