Daily Archives: September 14, 2017

IPFD crystals in lysosomes

RedI cut around the top end of the crystal inclusion in the image from the previous post, and erased (in photoshop) areas that allowed me to pseudocolor the objects that I could see in the lysosomal phase of this phagososome. I think the periodicity is going to be interesting with a 13-15nm round central dense area and a lucent area around that density (like a donut), still with a layer of dense protein or a trilaminar membrane.

It was fairly easy to see the repeating dots and lucent surroundings emerge as a repeating pattern along the terminal membrane at the long axis of the IPFD inclusion. IPFD crystals show for the most part a definite polarity, and any substructure in the IPFD inclusion itself is see on the butt ends of the short width axis, while a very smooth bounding area is seen on both sides running in a parallel direction.

Below, same pix as 9 13 2017 post but with a bounding box which aligns to the inset image below again.


Red dot=ribosome at 27nm diameter, purple dot=central densities, tangential end sections, 40nm dimension for a cross sectional diameter of the electron lucent area surrounding the purple dot, and 13 nm dimension is for the purple dots (some larger some smaller).