12 dodecamers of SP-D: Peak 5 height and valley (grayscale 0-255)
12 dodecamers of SP-D: Peak 4 height and valley (grayscale 0-255)
Histograms: glycosylation peak height – 12 dodecamers
It is easy to see where some images of SP-D dodecamers are not as well defined in terms of peak number as others. Peaks to the left of the mean, meadian, are found here. Kudos to the makers of calculator.net and gigacalculator.com. Being a visual person, their Q-Q plots, histograms and various little round indicators of significance and especially calculator.net, their explanations, oh and the Shapiro-Wilks calculator. I thank you all.
12 dodecamers of SP-D: Tiny peak height and valley (grayscale 0-255)
This very thin and small peak is detected about 20% of the time on the downslope of the very large N term peak. I checked to see whether the image processing peak finding was any different in terms of detection than the combined image and signal processing functions and they were virtually identical (missing 79% of the time, detected 21% of the time).
12 dodecamers of SP-D: N term peak height and valley (grayscale 0-255)
Same layout and procedures as before. N term peak height data from 12 dodecamers of SP-D. Grayscale values for peak height and valley determined using ImageJ. N of 752 is the total number of trimers in 12 dodecamers using dozens of images, image filters, and signal processing functions. INdividual dodecamers listed as well as a summar.
12 dodecamers of SP-D: neck peak and adjacent carbohydrate recognition domain peak width (peaks 7 and 8)
12 dodecamers of SP-D: neck peak and adjacent carbohydrate recognition domain peaks (peaks 7 and 8). images below are in the same basic format. Coiled coil neck peak width, and the peak width of the carbohydrate recognition domain.
DIfferent numbers of data in columns from the neck peak to the CRD peak are the result of the fact that the CRD peak is present 100% of the time, while the neck peak is seems to be present (as in not detected because it is likely folded under one of the CRD domain’s globby structure. I calculated including the undetected (as 0) and deleted the 0’s and recalculated).
The new American Dream: uselessness, fantasy, unearned wealth, unmerited power
The new American Dream: I run a pattern business, stained glass patterns, cross stitch patterns, peyote stitch beading patterns. I found some images of my patterns skimmed by a site called “second life”, they were valentine pictures, goodness, each sold (or brought value) of $100 of their “whatever” currency.
Of course there was no comment about where they came from, or reference to where to buy the patterns, they were just used without permission. Then came a thought, of course. They are living the ——-
new american dream
uselessness – fantasy – unearned wealth – unmerited power
12 dodecamers of SP-D: peak 6., just before the coiled coil neck peak
12 dodecamers of SP-D: peak 6., just before the coiled coil neck peak – this peak occurs much of the time, but not 100pc. number of missing (undetected) peaks in the area of peak 6 is shown in the line for mode… with a zero and parenthesis of the number of absences). In several cases a dodecamer would show a peak in the area of peak 6, 100% of the time. These columns of statistics will NOT have an adjacent column of statistics (top is yellow) correlating with the values MINUS the undetected peaks (e.g. the first two and the fourth dodecamers on the second row of data have no column with a yellow header, thus all measured hexamers had peak 6 on each plot.
12 dodecamers of SP-D: glycosylation peak and adjacent peaks (peaks 4 and 5)
The approach to the statistics is the same as has been used before. The summary has two elements if there are any missing values. if there are none, or as in the case of peak 4, only one or two out of several hundred, then i have just left them in. If the missing values (see two bottom sets of plots that are the values found for peak 5 are several percent of the total (recall… peak counting here begins at the N term and moves left to the CRD and width is reported in nm, as determine for each image by the bar marker provided by the author — which… can vary btw) , then there are four summar columns (one set with missing values calculated in as “0”s, and one set where they are left out). In these data, peak 4 (adjacent and lateral to the glycosylation peak in SP-D) is about 13 nm in width at the valley, while the peak adjacent to peak 4 (peak 5) is less than half that wide at 5.7 nm.
