Daily Archives: June 17, 2016

5 year old mongrel dog lung RER inclusion ???

Layered intracisternal protein granules in mammalian lung, Ultimate order, the cell

These accumulations are found in the peripheral lung of a 5 year old dog that was part of research for an entirely different topic.  The identification of the cell was not really possible, there were not absolute defining features, except there were mitochondria, perhaps a lipid droplet (cell membrane is obscure around this area), dense coated endocytic vesicles,  rough endoplasmic reticulum, and it is adjacent to a cell(s) with microvilli, basement membrane, and alveolar space. What it is, I have no clue.

neg 9931, blcok 24669, dog 5 yr, original mag 21750 x 4 pole piece V seimens 1A

dog_lung_prot

Early efforts at morphometry :)

Rants, Ultimate order, the cell

Long ago, my daughter was frequently at work, and here, as reminded of by finding this micrograph in my stash, as a young child she must have been visiting my office – maybe school was on a “teacher’s” day, or some other issue was at hand.  Many of the methods for morphometry I have used (and developed) over the decades (to determine organelle sizes, incidence, etc) she must have been aware of.  Here she correctly outlines the nucleus, the cell plasma lemma, and in particular she liked the granules in the lower right (ha ha) I do not remember what APUD stood for that day.  This micrograph I must have given her to entertain her…and teach her that science can be fun, and also can be for women (believe me, when I was in graduate school it was not a happy place for women), and it can be artistic.  I don’t remember how old she was, but not very old.   The heart is “mine” in finding this memory.

Kids_in_science