Daily Archives: June 6, 2016

Surfactant protein D rotary shadowed fuzzy ball

Electron microgaphs of lung, Layered intracisternal protein granules in mammalian lung, surfactant proteins A and D, The cell: images, Ultimate order, the cell

Here is an awesome picture in a publication by the following individuals: Martin van Eijk, Chris H.A. van de Lest, Joseph J. Batenburg, Arie B. Vaandrager, Joseph Meschi, Kevan L. Hartshorn, Lambert M.G. van Golde, and Henk P. Haagsman “Porcine Surfactant Protein D Is N-glycosylated in its Carbohydrate Recognition Domain and Is Assembled into Differently Charged Oligomers”, American Journal of Respiratory Cell and Molecular Biology, Vol. 26, No. 6 (2002), pp. 739-747, which I have cropped and repositioned and added a micron marker to.

I am trying to sort out whether the banded protein in the RER of some guinea pig, ferret, and mongrel dog specimens that I have repeatedly observed could be surfactant protein A or perhaps surfactant protein D.  It seems to me that It is possible for the 100 nm periodicity with a central dark band to be surfactant protein D, but because of the relative abundance of SP-A in lung, it seems less likely, though not impossible that it is SP-D.  Another study by: Stefanie M Heinrich and Matthias Griese Assessment of Surfactant Protein A (SP-A) dependent agglutination BMC Pulmonary MedicineBMC series,  shows artificial organization of SP-A, which does the mirror aggregation as I have predicted in my images.  They used streptavidin beads in vitro to accomplish this, and while not showing images that I particularly “believed” the diagram they provided was similar to mine.

Upper image the cruciform tetramer, bottom image the SP-D fuzzy ball.

I think SP-D may not be the protein in the intracisternal RER (granules) profiles in guinea pig in my micrographs, because of the low electron density of the space between the CRDs, that similar area in the SP-A showing a greater relative electron density than seen in the fuzzy ball below.  This is also more reminiscent of the periodicity seen in the langrin/CD 207 protein in Langerhans cells (Birbeck granules), as you will recall that that linear density is electron pale and very electron dense, not more middle density like the intracisternal protein in guinea pig type II cells in these posts.

surfactant_D_rotary_shadowed_SP-D

 

Kroger company — where is your integrity

Rants, Ultimate order, the cell

I need to sit on my “thoughts” and “obsessions” (LOL) keep them under wraps, navigate out of situations that cause me stress, keep my peace, find alternatives to the “big boxes” and support the independent and wise business owners who do their own thing.
The object of my stress yesterday just really was silly.  I have shopped Kroger for many decades, but no more. Their recent rebranding of lesser quality foods as “simple truth” makes me wretch, I check the labels and their “simple truth” is just a marketing scam.  Who takes a brand of peanut butter, adds more sugar, then sells it with a “green” label under the title of “simple truth”… no one with integrity does that.
So on the way out of the Kroger parking lot last weekend I call the number on my receipt and rattle off the long list of grievances I have against the company, including their refusal to call me back with a solution to defective (and potentially increasing the risk of food-born illnesses) packaging, after repeated calls, and after asking the little girl who was on the phone to please act like a concerned empolyee and make a comment to the Kroger headquarters she said she would “not” because she “liked” the way Kroger –!!!  I dare say, I rolled my eyes and immediately said to myself…. well “there you have it”  if this is what the people want, then I need to change where I shop.
I did go online and write up my complaints on the “how are we doing” Kroger site, we will see if they ignore… well I don’t need to ask.  If Kroger is trying to emulate K-Mart, Walmart, big box, will they are doing it.  Ha ha. But it becomes clear that they don’t care about the health of their customers, just for their profit– and the right hand feedeth the left… ha ha… the pharmacy for the heart disease, type II diabetes, high blood pressure, and obesity are right in the same store.             Are we blind, deaf and dumb?