“Desmosomes are a complex assembly of protein molecules that form at the cell surface and mediate cell–cell adhesion. Much is known about the composition of desmosomes and there is an established consensus for the location of and interactions between constituent proteins within the assembly. Furthermore, X-ray crystallography has determined atomic structures of isolated domains from several constituent proteins. Nevertheless, there is a lack of understanding about the architecture of the intact assembly and the physical principles behind the adhesive strength of desmosomes therefore remain vague. We have used electron tomography to address this problem. In previous work, we investigated the in situ structure of desmosomes from newborn mouse skin preserved by freeze-substitution and imaged in resin-embedded thin sections. In our present work, we have isolated desmosomes from cow snout and imaged them in the frozen unstained state. Although not definitive, the resulting images provide support for the irregular groupings of cadherin molecules seen previously in mouse skin.” ABSTRACT from Gethin Rh. Owen, Devrim Acehan, K.D. Derr, William J. Rice, David L. Stokes . Some things might be right, others not…. the last idea that cadherin molecules are grouped irregularly i don’t like. (This article i wont read as it is pay-per-view, which really galls me particularly because most research is paid for by tax-dollars and should be free).

” I found a dimension for the width “wide intercellular space (~240 A)” at 24nm and a measurement of the intermediate filament at 8nm (i presume diameter). The 22-24nm (could be less in some instances more like 15nm) as the width of the desmosomal intercellular space (which includes the densities on the outer leaflet of the plasmalemma from both cells and the central dense line of the desmosome.  That measurement looks about right. From this I estimate the width of the electron lucent annulus of the desmosome (which is much more evident when there is a tight junction on either side of the desmosome) to be dependent upon where the section sectioned the spot desmosome, and whether the cut was equatorial or tangent to the spot itself. On one micrograph (stub tail monkey hepatocyte) in which one area of the desmosome is close to a tight junction, shows this lucent area (the outer annulus) to be about 35nm in its radius – that dimension applies to the edge of the central dense line extending to the tight junction. The width of that particular desmosome (where the central line was apparent) was @265nm.  This makes the ratio of the dense desmosomal central line density distance to lucent annulus distance in cross section about 3.5%.

That lucent area may not be a uniform annulus, but if it were even, this assumes pretty much a “round” desmosomal spot weld. In measuring the radius of the annulus (just in the part ring part) I understand that the central dense line of the desmosome will appear in any view with the spot perpendicular to the plane of section, and this doesn’t diminish the fact that closer cuts to the outer edge of the desmosome will have a greater annulus to spot ratio. Fuzzing of the central dense line in the desmosome occurs when the angle of the section is not perpendicular to the spot, and can happen either at an equatorial section or anywere past that equator. So. any question I can ask, someone has an answer to…. so here is the definition of calculating the area of an annulus.. ha ha “An annulus (Latin word for ring) is a two-dimensional region of space bounded by two concentric circles. The area of an annulus is the difference in the areas of the larger circle of radius R, and the smaller circle of radius r. Mean radius (ρ) is the average of the exterior (R) and interior (r) radii. Breadth (δ) is the width of the annulus. “ So I just have to figure out how to use the online calculator.

The ratio of lucent annulus to dense desmosome is not too apparent when there is no tight junction visible right next to it. But that said, I can see a slight increase in plasmalemmal density for a short distance (same type of annulus arrangement as if there were a tight junction there) on each end of the desomosome cross section.