Monthly Archives: June 2016

Bisphenol A: straining of gnats swallowing camels?

toxi-city_4I appreciate this site (linked below) and its comments on BPA, as it sort of sums up what I have read about bisphenol A over the last 6 years.  When we find something so entrenched in daily life as plastic might not be “good for us” it takes a huge amount of effort to effect small changes to eliminate it. This means change on the consumer side (comfort and convenience) which is hard to deny, and voluntary change on the side of the producers (profit and greed) which is very unlikely to happen.chemical_engineering_news

http://cen.acs.org/articles/89/i23/Exposure-Routes-Confound-BPA-Debate.html

Some specific articles are here:

1) Bisphenol A  2) Debating BPA’s toxicity 3) BPA is indespensible for making plastic 4) Exposure Routes Confound BPA Debate

I debate daily in my own mind as to what is the greater risk to my health, the negative effects of the “stress” worrying about exposure to PBA. or the biological effect of the “toxin” and whether the effort exerted for “avoidance” is appropriate to the relative daily “dose” I receive, especially when taken into consideration with all other xenoestrogens to which I am exposed.

BPA manufacturers suggest that, given the level of exposure to phytoestrogens in the daily diet (aside here—-we all know that the vegetables which have phytoestrogens are NOT a part of most individuals daily diet (LOL)), the amounts of BPA that leach from polycarbonate plastics and ­epoxy resins may not really matter and in addition BPA is not the only chemical we encounter daily with estrogenic effects, nor does BPA have the highest estrogenic effect of these other chemicals, but one thing it is, is plentiful. It makes sense, because of the ease of contact, the abundance of the compound, the continuousness of our contact and the millions of pounds of BPA produced each year, that we give some small consideration to “limiting” our BPA exposure.

At some point (as happened for smokes, alcohol and drugs, lack of exercise, poor diet, etc etc) more will be known and more prudent measures applied.

Is there bisphenol A in my flexible metal kitchen faucet water supply lines?

How is it that we cannot learn from our mistakes, as a society. This little compound, bisphenol A, has been a mainstay of the economy for several decades but should it be protected? just because of its massive presence, or should we learn from our mistakes, like lead in gasoline, asbestos as a fire-retardant, believing that pesticides are a good measure for crop increase, and that plastics are inert.bisphenol_A

Consider the massive amount of evidence that was required before people who smoked actually believed that smoking was hazardous to their health. Consider too, the massive numbers of individuals that think that alcohol (because of its delayed onset of visible effects) is a safe and fun way to get a buzz. Consider, as well, that just because we don’t see blatant effects from absorbing the plasticizers in our every day plastic existence that they have no effect either.

HERE is verbatim what NIH – tox town has to say:

tox-town

Bisphenol A (BPA) en español

Bisphenol A (BPA) is used to make lightweight, hard plastics.

What is bisphenol A?

Bisphenol A, also called BPA, is a chemical that has been used since the 1960s to make lightweight, hard plastics and epoxy resins. In its pure form, it is a white solid with a medicinal odor. More than a billion pounds of BPA are produced in the United States every year.

BPA plastic products are usually clear and hard and are often called polycarbonate plastics. BPA plastic products include food and drink packaging, water bottles, infant and baby bottles, infant feeding cups, reusable cups, compact discs, automobile parts, impact-resistant safety equipment, plastic dinnerware, eyeglass lenses, toys, and medical devices. BPA epoxy resins are used as lacquers to coat metal products such as the inside lining of  metal food cans, bottle tops, wine vat linings, floorings, paints, and water supply pipes. Some flame retardants, dental sealants, and dental composites may also contain BPA. BPA is used in the recycling of thermal paper, such as receipts, self-adhesive labels, and fax paper. It is also used to make polyvinyl chloride plastics.

Plastic products made with BPA will have a #7 recycling symbol on them or contain the letters “PC” near the recycling symbol. BPA is not one of the phthalates, which are found in soft plastics.

How might I be exposed to bisphenol A?

Human exposure to bisphenol A is widespread, according to the National Toxicology Program. Most human exposure to BPA comes through food and beverages. You can also be exposed through air, dust, and water. BPA can leach into food from the linings of canned foods and from plastic products that are made with BPA. More BPA leaches from food and beverage containers if those foods and liquids are hot or boiling. If food containers or bottles are scratched or damaged, more BPA may be released into the food or liquid. The highest estimated exposures to BPA occur in infants and children, according to the National Toxicology Program.

At home, you and your family can be exposed to BPA if you use plastic food containers, canned foods, water or baby bottles, plastic dinnerware, reusable cups, and other consumer products that are made with BPA. You can have short-term exposure to BPA following the application of certain dental sealants that are made with BPA materials.

At work, you can be exposed to BPA by inhaling it or having skin contact with it if you work at a facility that manufactures BPA or products that are made with it.

How can bisphenol A affect my health?

Bisphenol A is an endocrine disruptor, which is a chemical that may interfere with the production or activity of hormones in the human endocrine system. According to the World Health Organization’s International Programme on Chemical Safety, there is still uncertainty about some links between human health effects and exposure to endocrine disruptors.

Both the U.S. Food and Drug Administration (FDA) and the National Toxicology Program (NTP) have “some concern” for effects on the brain, behavior, and prostate gland in fetuses, infants, and children at current human exposures to BPA. The NTP has “minimal concern” for effects on the mammary gland and an earlier age for puberty for females, in fetuses, infants, and children at current human exposures to BPA. The possibility that BPA may alter human development cannot be dismissed, according to the NTP. In cooperation with the NTP, FDA is carrying out in-depth studies to answer key questions and clarify uncertainties about the risks of BPA.

In the workplace, exposure to BPA dust may irritate the eyes, make skin sensitive, and cause dermatitis, irritated skin, and eczema. Contact with BPA may burn the eyes, lips, and skin. Inhaling BPA can irritate the nose and throat and cause coughing and wheezing. Exposure can cause headache, nausea, abdominal pain, and vomiting.

More research about BPA is needed to understand exactly how current findings relate to human health and development, according to the U.S. Food and Drug Administration and the National Toxicology Program.

If you think your health has been affected by exposure to BPA, contact your health care professional.

For poisoning emergencies or questions about possible poisons, contact your local poison control center at .

This description is based on the information found in the Web links listed with this topic.

You can look up their links if you like. In the list above they forgot to mention kitchen supply lines !!!!!

MY NEW KITCHEN WATER SUPPLY LINES ARE from Brasscraft (though touted as phthalate free) are braided metal LINED WITH flexible PVC… they will increasingly (with age) give off BPA in my drinking water, more from the hot water tap than the cold water tap, but from both.  If i already to not eat canned foods (lined with flexible PVC containing BPA, why would I exponentially increase the amount of BPA i consume every day by putting this in my own kitchen plumbing. 

When investigating the metallic taste in the water I have coming out of my new Moen kitchen faucet I began investigating the source, and trying to eliminate it, and I found out many things about current plumbing fixtures, lines, valves, cartridges, and dopes that I really did not like.

For starters, I had copper, now i cannot replace it, why?? no longer made in 3/8 inch.  I must either use all PVC flexible line, or braided metal lined with PVC or brass.  I must now investigate what is going to be leaching out of the brass lines….  I know we can’t always know everything, its a huge task to even take in the changes we must, but I am really overwhelmed when I ask Moen, or Home Depot, or Brasscraft, or the plumber and they all say,  well, I smoke, and I drink, and i have flexible woven supply lines with PVC,  and i am just fine.   ha ha…

Metallic tasting water from my redone kitchen plumbing !!!

Woe to the world, technology trashes us all, it goes ahead with products and innovations which have clearly never been tested for 1) durability (except maybe for planned obsolescence ha ha) 2) consumer inconvenience (except possibly just to create discord and irritation), 3) ease of installation (while charging more and providing less), and with abandon as applies to any health concerns or inconveniences to the customer.

Case in point: the unfortunate but simple process of changing out a faucet in my kitchen has taken me tens of hours in headache and in research on the internet to find one simple answer.  What is making my water taste metallic.  So after searching the internet there is not much to learn, after calling the companies who made the flexible supply lines i find that the plastic they used is PVC, which we all know will leach out bisphenol A.  Not just the necessary 3 inches of flexible piping, but the coiled way too long excess of 12 inches (what gives). So first on the agenda is to have someone come back to remove the new supply lines and put copper back in (which was there originally)

Next headache is that Moen doesn’t know or can’t tell me what the ** their faucets are made of– some kind of zinc (no doubt containing a considerable amount of contaminating cadmium) mixed with magnesium (i don’t mind that) and the copper is fine but the lead is not OK,  Moen did tell me that they have to meet or exceed California requirements for % of the brass that can be lead which (thank you California) is less than many other states. So the valves are brass… but the cheap faucet that I bought is probably mostly cheap zinc, which includes other metals.

What goes into the shut off valves.

How does the regular “joe” take care of all that the world dumps on them without feeling crazy.  These things should be part of the conscience of every business, and every person.

A test obtained from the Cincinnati Water Works, takes a week to obtain, and 30 days to get results… ha ha…  this is crazy, since after 30 days of drinking water from new faucet, two feet of supply lines and new valves…well, that is just not acceptable.

Surfactant protein D rotary shadowed fuzzy ball

Here is an awesome picture in a publication by the following individuals: Martin van Eijk, Chris H.A. van de Lest, Joseph J. Batenburg, Arie B. Vaandrager, Joseph Meschi, Kevan L. Hartshorn, Lambert M.G. van Golde, and Henk P. Haagsman “Porcine Surfactant Protein D Is N-glycosylated in its Carbohydrate Recognition Domain and Is Assembled into Differently Charged Oligomers”, American Journal of Respiratory Cell and Molecular Biology, Vol. 26, No. 6 (2002), pp. 739-747, which I have cropped and repositioned and added a micron marker to.

I am trying to sort out whether the banded protein in the RER of some guinea pig, ferret, and mongrel dog specimens that I have repeatedly observed could be surfactant protein A or perhaps surfactant protein D.  It seems to me that It is possible for the 100 nm periodicity with a central dark band to be surfactant protein D, but because of the relative abundance of SP-A in lung, it seems less likely, though not impossible that it is SP-D.  Another study by: Stefanie M Heinrich and Matthias Griese Assessment of Surfactant Protein A (SP-A) dependent agglutination BMC Pulmonary MedicineBMC series,  shows artificial organization of SP-A, which does the mirror aggregation as I have predicted in my images.  They used streptavidin beads in vitro to accomplish this, and while not showing images that I particularly “believed” the diagram they provided was similar to mine.

Upper image the cruciform tetramer, bottom image the SP-D fuzzy ball.

I think SP-D may not be the protein in the intracisternal RER (granules) profiles in guinea pig in my micrographs, because of the low electron density of the space between the CRDs, that similar area in the SP-A showing a greater relative electron density than seen in the fuzzy ball below.  This is also more reminiscent of the periodicity seen in the langrin/CD 207 protein in Langerhans cells (Birbeck granules), as you will recall that that linear density is electron pale and very electron dense, not more middle density like the intracisternal protein in guinea pig type II cells in these posts.

surfactant_D_rotary_shadowed_SP-D

 

Kroger company — where is your integrity

I need to sit on my “thoughts” and “obsessions” (LOL) keep them under wraps, navigate out of situations that cause me stress, keep my peace, find alternatives to the “big boxes” and support the independent and wise business owners who do their own thing.
The object of my stress yesterday just really was silly.  I have shopped Kroger for many decades, but no more. Their recent rebranding of lesser quality foods as “simple truth” makes me wretch, I check the labels and their “simple truth” is just a marketing scam.  Who takes a brand of peanut butter, adds more sugar, then sells it with a “green” label under the title of “simple truth”… no one with integrity does that.
So on the way out of the Kroger parking lot last weekend I call the number on my receipt and rattle off the long list of grievances I have against the company, including their refusal to call me back with a solution to defective (and potentially increasing the risk of food-born illnesses) packaging, after repeated calls, and after asking the little girl who was on the phone to please act like a concerned empolyee and make a comment to the Kroger headquarters she said she would “not” because she “liked” the way Kroger –!!!  I dare say, I rolled my eyes and immediately said to myself…. well “there you have it”  if this is what the people want, then I need to change where I shop.
I did go online and write up my complaints on the “how are we doing” Kroger site, we will see if they ignore… well I don’t need to ask.  If Kroger is trying to emulate K-Mart, Walmart, big box, will they are doing it.  Ha ha. But it becomes clear that they don’t care about the health of their customers, just for their profit– and the right hand feedeth the left… ha ha… the pharmacy for the heart disease, type II diabetes, high blood pressure, and obesity are right in the same store.             Are we blind, deaf and dumb?

Concentric organization of protein layered in an RER profile presumed to be surfactant protein A

Guinea pig, aged, routine electron microscopy, type II alveolar cell, in particular the RER and a highly organized protein contained: possibly surfactant protein A.

This micrograph is one of the few that is concentric and shows banding at the same time, at least that I have encountered.  The center period looks like the others which are only a single set of bands, i.e. a single central dark area, a lighter band, and then the outer band (which would include the CDR part of the protein octadecamers and the RER membrane.

It has ribosomes for size comparison, at 20-30 nm in diameter, and as can be seen from the longitudinal profile of RER in the upper left corner where the banding is more obvious, even in the concentric arrangement, the spacing of a single complete period is about 100 nm. I evened out the exposure of this micrograph, nothing else.

round_layered_ICB_gp

Longitudinal and cross sections of single period of SP-A? protein in guinea pig alveolar type II cell

From the same micrograph here are two tiny sections of the larger image which represent a well demarcated cross section (on the right) and a longitudinal section (on the left) where the organization of this protein is parallel to the RER membrane.  The banding in each is evident, however the bottom pair of images is “as is” and the top pair i have used the “burn” tool in photoshop to highlight where in the cross section the bands that match those in the longitudinal section batch up, and have added arrows as well.  One arrow to the central dot (which corresponds to the inner more dense band, presumably where the carbohydrate recognition domains in the trimer (or octadecamer) lie, and to the lighter band which may correspond to the N terminal regions.  It is my opinion that the N terminal groupings of the octadecamer come together in the highly periodic, but lighter bands, mirrored in the longitudinal section.

It is not clear how the inner CRDs fit into the central dot of the cross section…  particularly since the cross section is about 100 nm in diameter.  Picture on left has three or four ribosomes to compare size  (somewhere between 20-30 nm diameter) picture on right has one ribosome to compare.  Both are identical magnifications and imaging treatments.

cross_longitudinal_single_period_gp